Intracellular Thermometry with Fluorescent Molecular Thermometers

Written for an audience of researchers and professionals in the bioanalytical sciences, this comprehensive overview on intracellular temperature sensing describes fundamentals, applications and future perspectives of this promising new technology.

Inhaltsverzeichnis:

1 Temperature in Biological Species
1.1 Temperature in Our Life
1.2 Body Temperature
1.3 Effects of Environmental Temperature on Biological Function
1.4 Molecules Associated with Biological Responses to Environmental Temperature

2 Development of Fluorescent Molecular Thermometers
2.1 Physical Chemistry of Fluorescence
2.2 Fluorescent Molecular Thermometers
2.2.1 Small Organic compounds involving intersystem crossing
2.2.2 Small Organic Compounds Involving Conformational Change
2.2.2 Organometallic Complexes
2.2.3 Excimers and Exciplex
2.2.4 Host-Guest Complexes
2.2.5 Thermoresponsive Macromolecules
2.2.6 Inorganic Materials
2.2.7 Others

3 Intracellular Thermometry with Fluorescent Molecular Thermometers
3.1 Early Attempts for Cellular Thermometry
3.2 Introduction of Fluorescent Molecular Thermometers into a Living Cell
3.3 Cytotoxicity Assessment
3.4 Practice of Intracellular Thermometry with Fluorescent Molec ular Thermometers
3.5 Intracellular Thermometry with Organelle-targeted Fluorescent Molecular Thermometers
3.6 Intracellular Thermometry of Brown Adipocytes
3.7 Application of Intracellular Thermometry in Various Biological Fields

4 Cellular Thermometry Based on Non-fluorometric Principles
4.1 Infrared Thermometry
4.2 Photoacoustic Thermometry
4.3 Raman Thermometry
4.4 Use of Transmission Spectroscopy
4.5 Thermocouples
4.6 Resonant Thermal Sensors
4.7 Bimaterial Microcantilevers
4.8 Thermistors

5 Reliability of Intracellular Thermometry with Fluorescent Molecular Thermometers
5.1 Validity of a Calibration Curve
5.2 Possible Artifacts in Thermometry of Biological Samples
5.3 Difference between Experimentally Observed and Theoretically Calculated Intracellular Temperature Variation

6 The Future of Intracellular Thermometry
6.1 In vivo Thermometry
6.2 Thermal Medicine
6.3 Thermal Signaling
6.4 Absolute Determination of Intracellular Temperature
6.5 Simultaneous Measurement of Multiple Parameters
6.6 Intracellular Thermometry of Plant Cells

Appendix
A.1 Review and account items on fluorescent molecular thermometers
A.2 Comprehensive collection of fluorescent polymeric thermometers based on the combination of a thermoresponsive polymer and an environment-sensitive fluorophore
A.3 Comprehensive collection of fluorescent nanogel thermometers based on the combination of a thermoresponsive polymer and an environment-sensitive fluorophore
A.4 Non-covalent mixtures of a thermoresponsive polymer and an environment-sensitive fluorophore
A.5 Fluorescent logic gates based on the combination of a thermoresponsive polymer and an environment-sensitive fluorophore
A.6 Fluorescent inorganic thermometers